The ultimate goal of nanotechnology is to change the functionalities of devices by modulating individual atoms, which needs the characterization and identification of each site of the whole system in three dimension. The recent development of electron microscopy based imaging techniques such as Atomic electron tomography and Electron ptychography enable us to see the individual atoms in two and three dimension without the assumption of crystallinity. Our lab is at the frontiers of electron imaging methods to not only correlate the 3D atomic structure and properties of different materials, but also monitorng the dynamic process in atomic resoltion with different conditions such as heating and electro-chemistry among time.